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The following table shows how the stock solutions are mixed to make up the phosphate-buffered Krebs-Ringer solution.
| Solution 1 |
0.90% NaCl |
100
parts |
| Solution 2 |
1.15% KCl |
4
parts |
| Solution 3 |
1.22% CaCl2 |
3
parts |
| Solution 4 |
3.82% MgSO4.7H2O |
1
part |
| Solution 5 |
0.1 M phosphate
buffer |
20
parts |
|
(The
phosphate buffer is made up by adding 0.1 mole of Na2HPO4
and 20 ml of 1M HCl to 1 litre of water.)
|
|
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