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In the remaining part of this
practical we consider one of the more commonly used physiological
solutions, the phosphate-bufferred Krebs-Ringer solution.
When preparing Krebs-Ringer solutions it is common practice
to prepare stock solutions of the appropriate chemicals which can
then be mixed together to produce the final solution. The following
table shows the stock solutions frequently used for making up phosphate-buffered
Krebs-Ringer solution.
Solution 1 |
0.90% NaCl |
Solution 2 |
1.15% KCl |
Solution 3 |
1.22% CaCl2 |
Solution 4 |
3.82% MgSO4.7H2O |
Solution 5 |
0.1 M phosphate buffer |
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